A Comparison of a rapid test for influenza with laboratory-based diagnosis in a paediatric population
DOI:
https://doi.org/10.33321/cdi.2005.29.26Keywords:
influenza, rapid tests, Directigen, point of care, diagnostic testingAbstract
The rapid and accurate detection of influenza A and B in a hospital setting is useful to confirm infection, exclude other diseases and assist in the management of patient illness including the possible use of specific antiviral therapy. We evaluated the use of the Directigen Flu A+B in a paediatric hospital laboratory in comparison with the established diagnostic tests direct immunofluorescence, viral culture and reverse transcriptase-polymerase chain reaction. A total of 193 respiratory specimens were examined and the Directigen test detected positive samples with an 80.8 per cent sensitivity and a specificity of 100 per cent. This study confirms other paediatric studies which have found the Directigen Flu A+B to be less sensitive than traditional laboratory tests but nevertheless to have a potential role in patient management especially when a positive result is obtained. Commun Dis Intell 2005;29:272–276.
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References
Simonsen L. The global impact of influenza on morbidity and mortality. Vaccine 1999; 17 Suppl 1:S3–S10.
Menec VH, Black C, MacWilliam L, Aoki FY. The impact of influenza-associated respiratory illnesses on hospitalizations, physician visits, emergency room visits, and mortality. Can J Public Health 2003;94:59–63.
Izurieta HS, Thompson WW, Kramarz P, Shay DK, Davis RL, DeStefano F, et al. Influenza and the rates of hospitalization for respiratory disease among infants and young children. N Engl J Med 2000;342:232–239.
Neuzil KM, Mellen BG, Wright PF, Mitchel EF Jnr, Griffin MR. The effect of influenza on hospitalizations, outpatient visits, and courses of antibiotics in children. N Engl J Med 2000;342:225–231.
Bonner AB, Monroe KW, Talley LI, Klasner AE, Kimberlin DW. Impact of the rapid diagnosis of influenza on physician decision-making and patient management in the pediatric emergency department: results of a randomized, prospective, controlled trial. Pediatrics 2003;112:363–367.
Storch GA. Rapid diagnostic tests for influenza. Curr Opin Pediatr 2003;15:77–84 [Review].
Pachucki CT. Rapid tests for influenza. Curr Infect Dis Rep 2005;7:187 –192.
Rodriguez WJ, Schwartz RH, Thorne MM. Evaluation of diagnostic tests for influenza in a pediatric practice. Pediatr Infect Dis J 2002;21:193–196.
Chan KH, Maldeis N, Pope W, Yup A, Ozinskas A, Gill J, et al. Evaluation of the Directigen Flu A+B test for rapid diagnosis of influenza virus type A and B infections. J Clin Microbiol 2002;40:1675–1680.
Reina J, Padilla E, Alonso F, Ruiz De Gopegui E, Munar M, Mari M. Evaluation of a new dot blot enzyme immunoassay (Directigen flu A+B) for simultaneou s and differential detection of influenza A and B virus antigens from respiratory samples. J Clin Microbiol 2002;40:3515–3517.
Ruest A, Michaud S, Deslandes S, Frost EH. Comparison of the Directigen Flu A+B Test, the QuickVue influenza test, and clinic al case definition to viral culture and reverse transcription-PCR for rapid diagnosis of influenza virus infection. J Clin Microbiol 2003;41:3487–3493.
Cazacu AC, Chung SE, Greer J, Demmler GJ. Comparison of the Directigen Flu A+B membrane enzyme immu noassay with viral culture for rapid detection of Influenza A and B viruses in respiratory specimens. J Clin Microbiol 2004;42:3707–3710.
Landry ML, Cohen S, Ferguson D. Comparison of Binax NOW and Directigen for rapid detection of influenza A and B. J Clin Virol 2004;31:113–115.
Alexander R, Lamb D, White T, Wentzel S, Politis J, Rijnsburger D, et al. 'RETCIF': a rapid, sensitive method for detection of viruses, applicable for large numbers of clinical samples. J Virol Methods. 2001;97:77–85.
Poddar SK. Influenza virus types and subtypes detection by single step single tube multiplex reverse transcription-polymerase chain reaction (RT–PCR) and agarose gel electrophoresis. J Virol Methods 2002;99:63–70.
Dunn JJ, Gordon C, Kelley C, Carroll KC. Comparison of the Denka-Seiken INFLU A.B-Quick and BD Directigen Flu A+B Kits with direct fluorescent-antibody staining and shell vial culture methods for rapid detection of influenza viruses. J Clin Microbiol 2003;41:2180–2183.
Woo PCY, Chiu SS, Seto WH, Peiris M. Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients. J Clin Microbiol 1997;35:1579–1581.
Boivin G, Cote S, Dery P, De Serres G, Bergeron MG. Multiplex real-time PCR assay for detect ion of influenza and human respiratory syncytial viruses. J Clin Microbiol 2004;42:45–51.
Neuzil KM, Zhu Y, Griffin MR, Edwards KM, Thompson JM, Tollefson SJ, et al. Burden of interpandemic influenza in children younger than 5 years: a 25-year prosp ective study. J Infect Dis 2002;185:147–152.
Centers for Disease Control and Prevention. Update: Influenza Activity— United States and Worldwide, 2003–04 Season, and Composition of the 2004–05 Influenza Vaccine. MMWR Morb Mortal Wkly Rep 2004;53:547–552. Available from: http:// www.cdc.gov/mmwr/preview/mmwrhtml/mm5325a1.htm
Milne BG, Williams S, May MLA, Kesson AM, Gillis J, Burgess MA. Influenza A associated morbidity and mortality in a paediatric intensive care unit. Commun Dis Intell 2004;28:504–509.
Sharma V, Dowd D, Slaughter AJ, Simon SD. Effect of rapid diagnosis of influenza virus type A on the emergency department management of febrile infants and toddlers. Arch Pediatr Adolesc Med 2002;156:41–43.
Poehling KA, Griffin MR, Dittus RS, Tang YW, Holland K, Li H, et al. Bedside diagnosis of influenza virus infections in hospitalized children. Pediatrics 2002;110:83–88.
Cazacu AC, Demmler GJ, Neuman MA, Forbes BA, Chung S, Greer J, et al. Comparison of a new lateral-flow chromatographic membrane immunoassay to viral culture for rapid detection and differentiation of influenza A and B viruses in respiratory specimens. J Clin Microbiol 2004;42:3661–3664.
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