The Molecular epidemiology of norovirus outbreaks in Victoria, 2014-15
DOI:
https://doi.org/10.33321/cdi.2017.41.5Keywords:
norovirus, outbreaks, genotypes, healthcare, non-healthcare, setting, RT-PCR, nucleotide sequencingAbstract
Noroviruses are a leading cause of outbreaks of gastroenteritis. This study examined the incidence and molecular characteristics of norovirus outbreaks in healthcare and non-healthcare settings in Victoria, Australia, over 2 years (2014–2015). Norovirus was detected in 65.7% and 60.4% of gastroenteritis outbreaks investigated for the years 2014 and 2015 respectively. There was a significant decline in the number of norovirus outbreaks in the period 2014 to 2015 although in both years norovirus outbreaks peaked in the latter part of the year. Norovirus Open Reading Frame (ORF) 2 (capsid) genotypes identified included GI.2, GI.3, GI.4, GI.5, GI.6, GI.9, GII.2, GII.3, GII.4, GII.6, GII.7, GII.8, GII.13 and GII.17. GII.4 was the most common genotype detected. In addition, the following ORF 1/ORF 2 recombinant forms were confirmed: GII.P4_NewOrleans_2009/GII.4_Sydney_2012, GII.P12/GII.3, GII.Pb (GII.21)/GII.3, GII.Pe/GII.2 and GII.Pe/GII.4_Sydney_2012. A significant decline was noted in the chief norovirus strain GII.Pe/GII.4_Sydney_2012 between 2014 and 2015 but there was a re-emergence of a GII.P4_ NewOrleans _2009 norovirus strain. Outbreaks involving the GII.P17/GII.17 genotype were also detected for the first time in Victoria. GI genotypes circulating in Victoria for the 2 years 2014 and 2015 underwent a dramatic change between the 2 years of the survey. Many genotypes could occur in both healthcare and non-healthcare settings although GI.3, GII.6, and GII.4 were significantly more common in healthcare settings. The study emphasises the complex way in which norovirus circulates throughout the community. Commun Dis Intell 2017;41(1):E21–E32.
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References
Green KY. Caliciviridae: the noroviruses. In: Knipe DM, Howley PM, eds. Fields Virology, 6th edn. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins, 2013. pp 582–608.
La Rosa G, Pourshaban M, Iaconelli M, Muscillo M. Detection of genogroup IV noroviruses in environmental and clinical samples and partial sequencing through rapid amplification of cDNA ends. Arch Virol 2008;153(11):2077–2083.
Aliabadi N, Lopman BA, Parashar UD, Hall AJ. Progress toward norovirus vaccines: considerations for further development and implementation in potential target populations. Expert Rev Vaccines 2015;14(9):1241–1253.
Lopman BA, Steele D, Kirkwood CD, Parashar UD. The vast and varied global burden of norovirus: prospects for prevention and control. PLoS Med 2016;13(4):e1001999.
Hall G, Kirk MD, Becker N, Gregory JE, Unicomb L, Millard G, et al. Estimating foodborne gastroenteritis, Australia. Emerg Infect Dis 2005;11(8):1257–1264.
Bernstein DI, Atmar RL, Lyon GM, Treanor JJ, Chen WH, Jiang X, et al. Norovirus vaccine against experimental human GII.4 virus illness: a challenge study in healthy adults. J Infect Dis 2015;211(6):870–878.
Kroneman A, Vega E, Vennema H, Vinje J, White PA, Hansman G, et al. Proposal for a unified norovirus nomenclature and genotyping. Arch Virol 2013;158(10):2059–2068.
Bull RA, Tanaka MM, White PA. Norovirus recombination. J Gen Virol 2007;88(12):3347–3359.
Bok K, Green KY. Norovirus gastroenteritis in immunocompromised patients. N Engl J Med 2012;367(22):2126–2132.
Fu J, Ai J, Jin M, Jiang C, Zhang J, Shi C, et al. Emergence of a new GII.17 norovirus variant in patients with acute gastroenteritis in Jiangsu, China, September 2014 to March 2015. Euro Surveill 2015;20(24):pii=21157.
Lu J, Sun L, Fang L, Yang F, Mo Y, Lao J, et al. Gastroenteritis outbreaks caused by norovirus GII.17, Guangdong Province, China, 2014–2015. Emerg Infect Dis 2015;21(7):1240–1242.
Matsushima Y, Ishikawa M, Shimizu T, Komane A, Kasuo S, Shinohara M, et al. Genetic analyses of GII.17 norovirus strains in diarrheal disease outbreaks from December 2014 to March 2015 in Japan reveal a novel polymerase sequence and amino acid substitutions in the capsid region. Euro Surveill 2015;20(26):pii=21173.
de Graaf M, van Beek J, Vennema H, Podkolzin AT, Hewitt J, Bucardo F, et al. Emergence of a novel GII.17 norovirus—End of the GII.4 era? Euro Surveill 2015;20(26):pii=21178.
Bruggink LD, Dunbar NL, Catton MG, Marshall JA. Norovirus genotype diversity associated with gastroenteritis outbreaks in Victoria in 2013. Commun Dis Intell 2015;39(1):E34–E41.
Lim KL, Hewitt J, Sitabkhan A, Eden J-S, Lun J, Levy A, et al. A multi-site study of norovirus molecular epidemiology in Australia and New Zealand, 2013–2014. PLoS One 2016;11(4):e0145254.
Witlox KJ, Karapanagiotidis T, Bruggink LD, Marshall JA. The effect of fecal turbidity on norovirus detection by reverse transcriptase polymerase chain reaction. Diagn Microbiol Infect Dis 2010;66(2):230–232.
Witlox KJ, Nguyen TN, Bruggink LD, Catton MG, Marshall JA. A comparative evaluation of the sensitivity of two automated and two manual nucleic acid extraction methods for the detection of norovirus by RT-PCR. J Virol Methods 2008,150(1–2):70–72.
Yuen LKW, Catton MG, Cox BJ, Wright PJ, Marshall JA. Heminested multiplex reverse transcription-PCR for detection and differentiation of Norwalk-like virus genogroups 1 and 2 in fecal samples. J Clin Microbiol 2001;39(7):2690–2694.
Bruggink LD, Witlox KJ, Sameer R, Catton MG, Marshall JA. Evaluation of the RIDA®QUICK immunochromatographic norovirus detection assay using specimens from Australian gastroenteritis incidents. J Virol Methods 2011;173(1):121–126.
McIver CJ, Bull RA, Tu ETV, Rawlinson WD, White PA. In: McIver CJ, ed. A compendium of laboratory diagnostic methods for common and unusual enteric pathogens– an Australian perspective. Norovirus and Sapovirus. Melbourne: The Australian Society for Microbiology Inc: 2005. pp 191–198.
Dunbar NL, Bruggink LD, Marshall JA. Evaluation of the RIDAGENE real-time assay for the detection of GI and GII norovirus. Diagn Microbiol Infect Dis 2014;79(3):317–321.
Bruggink LD, Oluwatoyin O, Sameer R, Witlox KJ, Marshall JA. Molecular and epidemiological features of gastroenteritis outbreaks involving genogroup I norovirus in Victoria, Australia, 2002–2010. J Med Virol 2012;84(9):1437–1448.
Bruggink LD, Dunbar NL, Marshall JA. Norovirus genotype diversity in community-based sporadic gastroenteritis incidents: a five year study. J Med Virol 2015;87(6):961–969.
Kroneman A, Vennema H, Deforche K, van der Avoort H, Penaranda S, Oberste MS, et al. An automated genotyping tool for enteroviruses and noroviruses. J Clin Virol 2011;51(2):121–125.
Remington RD, Schork MA. Statistics with applications to the biological and health sciences. Englewood Cliffs: Prentice-Hall Inc; 1970. pp 235–244.
Agresti A. Categorical data analysis. New York: John Wiley & Sons; 1990. pp 50–54.
Parra GI, Green KY. Genome of emerging norovirus GII.17, United States, 2014. Emerg Infect Dis 2015;21(8):1477–1479.
Lindesmith LC, Constantini V, Swanstrom J, Debbink K, Donaldson EF, Vinje J, et al. Emergence of a norovirus GII.4 strain correlates with changes in evolving blockade epitopes. J Virol 2013;87(5):2803–2813.
Lee C-C, Feng Y, Chen S-Y, Tsai C-N, Lai M-W, Chiu C-H. Emerging norovirus GII.17 in Taiwan. Clin Infect Dis 2015;61(11):1762–1764.
Bruggink L, Sameer R, Marshall J. Molecular and epidemiological characteristics of norovirus associated with community-based sporadic gastroenteritis incidents and norovirus outbreaks in Victoria, Australia, 2002–2007. Intervirology 2010;53(3):167–172.
Bruggink L, Marshall J. The relationship between health care and non-healthcare norovirus outbreak settings and norovirus genotype in Victoria, Australia, 2002–2005. J Microbiol Immunol Infect 2011;44(4):241–246.
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